Isolation, Purification and Characterization of Oil Degrading Bacteria from Different Oil Cake Samples Singh Poonam1,*, Kashyap Kumar1, Singh Satendra2 1Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agricultural, Technology and Sciences, Allahabad, India – 211007 2Department of Computational Biology and Bioinformatics, Sam Higginbottom Institute of Agricultural, Technology and Sciences, Allahabad, India – 211007 *Corresponding author: drpoonam.singh1@gmail.com
Online published on 10 June, 2015. Abstract Oil cakes of mustard and linseed were collected from Rambagh, Allahabad and Mahewa Gate, Allahabad. Two samples of mustard and one sample of linseed cake were taken as a sample for project. Bacteria were grown on these oil cake samples. From it different cultures were isolated and each was separately grown. The pure cultures grown were tested for oil degrading property. Those cultures, which showed oil degrading property, were selected and isolated. The cultures were then identified using Gram staining technique. A series of biochemical tests were done for the cultures consuming oil. The tests include starch hydrolysis test, urease production test and catalase production test. DNA was then isolated from these oil degrading cultures. Top Keywords oil cakes, bacterial growth, pure culture, oil degradation, gram staining. Top |