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Year : 2012, Volume : 36, Issue : 2
First page : ( 187) Last page : ( 191)
Print ISSN : 0250-4758. Online ISSN : 0973-970X. Published online : 2012 December 1.

Haemato-biochemical and pathological studies of alpha-hexyl cinnamic aldehyde toxicity in Wistar rats

Patel A.V.1, Joshi B.P.1, Undhad V.V.1, Dhaval F.T.1, Gupta Hemant1, Jivani B.M.1, Ghodasara D.J.1, Prajapati K.S.1,*

1Department of Veterinary Pathology, College of Veterinary Science and A.H., Anand Agriculture University, Anand-388001

*Correspondence author: email: kanti_prajapati@yahoo.com

Received:  17  July,  2012; Accepted:  13  September,  2012.

Abstract

The present study was conducted to evaluate the repeated dose dermal toxicity of alpha - hexyl cinnamic aldehyde (HCA) in male and female Wistar rats. The animals were divided into four different groups each comprising five male and five female rats. The group I served as control and was applied with isopropyl alcohol (2 ml/kg BW) while groups II, III, and IV were acted as treatment groups and HCA was applied topically at the dose rate of 170 mg/kg (low dose), 340 mg/kg (medium dose) and 680 mg/kg (high dose), respectively, for fourteen consecutive days. The clinical signs like erythema and hyperirritability were noticed in all the three treatment groups with lower severity in group II and highest in group IV rats. Among different haematological parameters studied, there was significant reduction in lymphocytes as well as significant increase in neutrophils, eosinophils and monocyte count in male and female rats belonged to group III and IV and no affect on erythrocyte related values. Significant decrease in total protein, albumin, glucose and triglyceride as well as increase in AST and ALT was observed in group IV rats as compared to control group. There was no alteration in kidney parameters such as creatinine, protein, sodium, potassium and chloride in treatment groups as compared to the control. There was rough, dry and thick skin along with crust formation in rats belonged to medium and high dose group. The histological lesions in the skin revealed thickening of epidermis, hyperkeratosis, hyperplasia of stratum germinativum, presence of necrotic debris in cornified layer and hyperplasia of sebaceous glands. The lesions were more pronounced in high dose group than in medium dose group.

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Keywords

Alpha-hexyl cinnamic aldehyde, Haemato-biochemical, Pathology, Wistar rats.

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