Effect of 2,4-d on embryogenic callus induction and evaluation of G418 on growth inhibition in rice calli Pathania Shivali, Sandhu Jagdeep Singh* School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana - 141004, Punjab *Corresponding author: js_sandhu@pau.edu
Online published on 12 April, 2021. Abstract An efficient in vitro callus induction study in rice genotypes Kitaake and PR124 was carried out using different concentrations of 2,4-D. Effect of 2,4-D was studied using four different concentrations in Kitaake (1, 2, 3, 4 mg/l) and in PR124 (1, 1.5, 2, 2.5 mg/l). This study found that Kitaake exhibited high frequency of callus induction on MS (Murashige and Skoog) medium supplemented with 2 mg/l 2,4-D, whereas in PR124, 1.5 mg/l concentration of 2,4-D was found to be the best for calli induction. Callus induction frequencies in Kitaake and PR124 were found to be 85 % and 64.4 %, respectively. Further, the study demonstrated that the growth of rice calli was not affected at lower concentrations of G418 and necrosis of calli occurred at 100 mg/l. These optimized concentrations of 2,4-D and G418 would be helpful to obtain desirable transformants in rice through Agrobacterium-mediated genetic transformation. Top Keywords Callus induction, 2, 4-D, G418, Genetic transformation, Kitaake, PR124. Top |