Indian Journal of Scientific Research
Year : 2017, Volume : 7, Issue : 2
First page : ( 7) Last page : ( 13)
Print ISSN : 0976-2876. Online ISSN : 2250-0138.

Induction of mutant strains of Trichoderma viride 1433 for biocontrol of Fusarium oxysporum f. sp. lycopersici

Chandra Subhash*

P. G. Department of Botany, Sri Murli Manohar Town P.G. College, Ballia, Uttar Pradesh, India

*Corresponding author

Online published on 21 May, 2018.

Abstract

Twenty two mutants of Trichoderma viride 1433 were obtained after treatment with NTG which were further screened against Fusarium oxysporum f. sp. lycopersici by dual culture method. All the mutants indicated high sporulation count and better growth patterns as compared to wild type but only four mutants showed enhanced antagonistic activity against Fusarium oxysporum f. sp. lycopersici in colony plate assay as compared with the wild type and control. Since mycoparasitism plays important role in antagonism mechanism of Trichoderma species, extracellular enzymatic activity of mutant strains was also assayed. The result of extracellular en zymatic activity showed that cellulase and β 1, 3-glucanase significantly increased in the mutant strains while protease activity was found suppressed in case of two mutant strains as compared to wild type. Among the mutant strains Tv m9 was found as most potent strain against Fusarium oxysporum f. sp. lycopersici, therefore, this strain can be studied in glasshouse experiment against the above pathogen.

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Keywords

Trichoderma viride 1433, Mutant Strains, Fusarium oxysporum f. sp. lycopersici.

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