(3.142.156.202)
Users online: 4645     
Ijournet
Email id
 

Indian Journal of Microbiology Research
Year : 2018, Volume : 5, Issue : 3
First page : ( 295) Last page : ( 298)
Print ISSN : 2394-546X. Online ISSN : 2394-5478.
Article DOI : 10.18231/2394-5478.2018.0062

Characterization of non albicans candida species and the antifungal susceptibility profile of clinical isolates

Balaraman Lekshmi1, Saritha N2,*, Raveendran Geetha3

1Senior Resident, Dept. of Microbiology, Government Medical College, KUHS, Thiruvananthapuram, Kerala

2Associate Professor, Dept. of Microbiology, Government Medical College, KUHS, Thiruvananthapuram, Kerala

3Professor and Head, Dept. of Microbiology, Government Medical College, KUHS, Kollam, Kerala, India

*Corresponding Author: Email: drsarithapradeep@gmail.com

Online published on 14 January, 2019.

Abstract

Introduction and Objectives

Antibiotics, immunodeficiencies and advances in medical practices have all contributed to increased incidence in fungal infections. In recent times Non albicans Candida species are increasingly being isolated from clinical specimens. The present study was aimed at the characterization and antifungal susceptibility of Non albicans Candida species from clinical specimens received in Microbiology Laboratory for one year from June 2015-May 2016.

Materials and Methods

A total of 181 Non albicans Candida isolates were included in the study. Candida was identified from clinical samples by gram stain, culture on Saboraud's Dextrose Agar (SDA) and germ tube test. The organisms were further speciated by cornmeal agar morphology, sugar assimilation and fermentation tests and growth on CHROM agar (Himedia). Antifungal sensitivity was performed by using disc diffusion method as per CLSI guidelines.

Results

The most common isolate was Candida tropicalis (59.11%) followed by C.glabrata (28.18%), C.parapsilosis (8.83%) and C.krusei (3.88%). Highest number of isolates were from urine (74/181). Resistance to Fluconazole in clinical isolates of C.tropicalis has increased. C.krusei was the most resistant species.

Conclusion

Use of CHROM agar is rapid, simple and cost effective compared to time consuming technically demanding expensive conventional methods. Antifungal susceptibility testing has to be carried out before starting therapy because of varying susceptibility pattern in different species of Candida.

Top

Keywords

Non albicans Candida species, Candidiasis, CHROM agar.

Top

 
║ Site map ║ Privacy Policy ║ Copyright ║ Terms & Conditions ║ Page Rank Tool
823,994,000 visitor(s) since 30th May, 2005.
All rights reserved. Site designed and maintained by DIVA ENTERPRISES PVT. LTD..
Note: Please use Internet Explorer (6.0 or above). Some functionalities may not work in other browsers.