Chitinase Production Ability of Trichoderma viride Mutants and Their Efficacy against Sclerotium rolfsii and Rhizoctonia bataticola Dandale Sujata*, Ingle S.T. Department of Plant Pathology, PGI, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra *Email: dandalesuju59@gmail.com
Online published on 2 January, 2017. Abstract Trichoderma viride are identified to act against on array of important soil borne plant pathogens. The antagonistic ability of a Trichoderma viride was determined by its physiological state so that change in genetical conditions could alter the antagonism. Hence, the genetic modification using mutagenesis offers the potential for producing improved bio-protection is likely to enhance their biocontrol capabilities against soil borne pathogen. For mutagenesis study the spores were exposed to chemical i.e. Ethyl Methyl Sulphonate (EMS) @ 100, 150, 200 and 250 μl/ml at time interval of 30, 45 and 60 minutes. Twelve Sclerotium rolfsii and Rhizoctonia bataticola mutants were obtained after chemical mutagenesis and tested for their antagonistic activities in vitro. Efficient mutants were tested uptosixgeneration to check theirstability. Among these, four stable mutantsviz., TVME 2b, TVME 2c, TVME 3b, TVME 3c and TVME 4c, TVME 4b were proved as effective antagonists against Sclerotium rolfsii, and TVME 3b, TVME 2b, TVME 3a, TVME 3c and TVME 2c were proved as effective antagonists against Rhizoctonia bataticola on the basis of their maximum bioefficiency. The highest chitinase enzyme units/mg of protein i.e. 0.64, 0.63, 0.63 and 0.60 was exerted in TVME2b, TVME2c, TVME3c and TVME4c. Top Keywords Mutation, Trichoderma viride, Ethyl Methyl Sulphonate (EMS), Chitinase, Sclerotium rolfsii, Rhizoctonia bataticola. Top |