The Protective Effects of Nigella Sativa (Ns), Nicotinic Acid (Na) and Zinc Sulphate (Zns) on Alcohol Induced Gastric Lesions in Albino Rats Abbas Mohamed F.1,, Hafez Essam M.2, Mohamed Ghada A.3, Belkhir Bashir A.4, Gdarah Khaled M.5 1Department of Forensic Medicine and Toxicology, El-Minia University, Egypt 2Department of Forensic Medicine and Toxicology, El-Minia University, Egypt 3Department of Forensic Medicine and Toxicology, El-Fayom University, Egypt 4Department of Forensic Medicine and Toxicology, El-Fateh University, Libya 5Department of Forensic Medicine and Toxicology, El-Fateh University, Libya *Email: kgdara@yahoo.co.uk
Abstract The present study was conducted to study the protective effects of zinc sulphate (ZnS), nicotinic acid (NA) and nigella sativa (NS) oil on alcohol induced gastric lesions in male albino rats. The study was carried out on 80 adult male albino rats weighing 200–250 g. The rats were divided into two equal groups; each group was subdivided into four subgroups (10 rats each). Group (A): which was subdivided into: subgroup (I): control group, subgroup (2): was intraperitoneally injected by ZnS, subgroup (3) was intraperitoneally injected by NA and subgroup (4): was orally administrated NS oil daily for two weeks. Group (B) which was subdivided into subgroup (I): rats were administrated ethanol 40% orally for two weeks, subgroup; (2): the rats were administrated ethanol 40% orally and intraperitoneally injected by ZnS, subgroup (3) the rats were administrated ethanol 40%orally and intraperitoneally injected by NA and subgroup (4) the rats were administrated ethanol 40% orally with NS oil orally daily for two weeks. After the last dose of each group, the animals were left for an hour then anaesthetized by ether. The gastric secretion was collected in the acute rat fistula. The abdomen was incised and both the stomach and the duodenum were exposed. The gastric secretion was collected for the analysis and sections were taken for histological examination. The results showed significant increase of gastric mucin and glutathione content and significant decrease in the volume of the gastric secretion, peptic activity, free acidity and gastric histamine content in ethanol induced gastric lesions when ZnS, NA or NS oil was used. Histopathological picture showed improvement of hemorrhage, edema and increase in the mucus layer thickness and regeneration of the gastric mucosal erosion. It is recommended to increase the administration of ZnS, NA or NS to patients with gastric ulcer. Top Keywords Nigella Sativa (NS), nicotinic acid (NA), sulphate (ZnS), gastroprotection, ethanol, Albino Rats. Top |