Influence of Glomus species on enhancement of primary and secondary metabolites in in vitro regenerated Drymaria cordata Indira M.N.1,*, Tejavathi D.H.2 1Department of Life Sciences, Kristu Jayanti College, Bangalore, Karnataka, India 2Department of Botany, Jnana Bharathi Campus, Bangalore University, Karnataka, India *Corresponding author e-mail: indiramn@gmail.com
Online published on 20 June, 2022. Abstract Drymaria cordata (L.) Willd. Ex Roem. & Schult. (Family: Caryophyllaceae) is one of the potential herb with multiple medicinal properties. The prime active principles which are therapeutically known are drymaritin and isovitexin. The present investigation was aimed to ascertain and quantify the primary and secondary metabolite content in in vitro regenerated Drymaria cordata when inoculated with Glomus species. The micro propagated plants were derived from nodal explants cultured on MS medium supplemented with BAP (4.44 μM), GA3 (1.44 μM) and 2,4-D (2.26 μM). Two species of Glomus namely Glomus mosseae and G. fasciculatum have been determined to noticeably enhance the production of metabolites in Drymaria cordata. Of the two bioinoculants, the primary and secondary metabolites have concomitantly increased in the in vitro regenerated plants inoculated with G. fasciculatum. Among the primary metabolites, an estimate of total chlorophyll (3.61±0.10 mg/g), carbohydrates (99.07±0.90 mg/g), reducing sugars (25.17±0.76 mg/g), protein content (56.60±0.61 mg/g) and amino acids (16.89±0.57 mg/g) were detected in the regenerated plants inoculated with G. fasciculatum. Plants treated with G. mosseae too have influenced in increasing the metabolite contents when compared to uninoculated plants. Maximum amount of phenolics (2.09±0.11 mg/g), alkaloids (27.90±0.85 mg/g), flavonoids (2.13±0.32 mg/g) and saponins (4.67±0.29 mg/g) was recorded in regenerated plants treated with G. fasciculatum in comparison to plants treated with G. mosseae. The present study potentially represents an alternative approach of promoting the metabolite content of this important medicinal plant. Top Keywords Drymaria cordata, In vitro, Regenerated, Glomus, Metabolites. Top |