Application of DNA-free CRISPR/Cas-mediated genome editing in crops: A review Kumari Varsha1,*, Kumawat Priyanka2, Rajput S.S.1, Yeri Sharanbasappa3, Gothwal D.K.1, Choudhary Sharda4, Kumhar B.L.1, Kunwar Ram1, Kumawat G.L.5 1Department of Plant Breeding and Genetics, Sri Karan Narendra Agriculture University, Jobner-303 329, Jaipur, Rajasthan, India 2Department of Agronomy, Sri Karan Narendra Agriculture University, Jobner-303 329, Jaipur, Rajasthan, India 3Department of Biotechnology, University of Agricultural Sciences, Raichur-584 104, Karnataka, India 4Department of Biotechnology, National Research Centre on Seed Spices, Ajmer-305 006, Tabiji, Rajasthan, India 5Department of Plant Pathology, Sri Karan Narendra Agriculture University, Jobner-303 329, Jaipur, Rajasthan, India *Corresponding Author: Varsha Kumari, Department of Plant Breeding and Genetics, Sri Karan Narendra Agriculture University, Jobner-303 329, Jaipur, Rajasthan, India, Email: varshagpb@gmail.com
Online published on 10 July, 2023. Abstract Clustered regularly interspaced short palindromic repeats/CRISPR associated nuclease 9 (CRISPR-Cas9) system was instigated first into eukaryotes in the last ten years are becoming productive and worldwide application for genome modification. Genome engineering through insertion of foreign DNA insert have numerous disadvantages which could be overcome by use of DNA-free genome editing. Various ways of DNA-free genome editing mediated by CRISPR/Cas systems are CRISPR/Cas delivery as ribonucleoprotein, delivery of CRISPR/Cas as virus-like particles and agrobacterium-based delivery of CRISPR/Cas. Crop improvement through DNA-free genome editing via CRISPR/Cas have been applied in rice, wheat, maize, tomato, soybean and rare species like Nicotiana benthamiana etc. It is method of choice for precise genome editing without genome shuffle in an organism. Top Keywords Cas9, CRISPR, Crop improvement, DNA-free-genome editing, Ribonucleoprotein. Top |