Comparison of anti-diabetic, anti-oxidant and anti-lipogenesis activity of Holarrhena antidysenterica plant parts Divya C.A.1,2, Dhar Sujan K.3, Anithkumar C.S.4, Das Manjula1,3,*, Shantaram Manjula2,** 1Tumor Immunology Program, Mazumdar Shaw Medical Foundation, 8th Floor MSMC, Narayana Health City, Bommasandra, Bangalore-560099, Karnataka, India 2Department of Studies and Research in Biochemistry, Mangalore University, Jnana Kaveri Post Graduate Centre, Chikka Aluvara, Kodagu-571232, Karnataka, India 3Beyond Antibody, In Cite Lab, MSMF, Narayana Health City, Bangalore-560099, Karnataka, India 4Vanaushadhi Seva Samste, Sidhalli Post, Kerehalli, Shivamogga-577419, Karnataka, India *Corresponding authors: Professor of Biochemistry, Mangalore University, Mangalagangotri-574199, Karnataka, India, Email: manjula59@gmail.com
**Corresponding author e-mail: manjula.msmf@gmail.com
Online published on 20 June, 2022. Abstract In this study we reported the development of an extensively characterized (end point assays) insulin-resistant in vitro model and comparison of anti-diabetic, anti-oxidant and anti-lipogenesis activity of Holarrhena antidysenterica (HA) leaf and bark, each extracted with 7 different solvents using the in vitro model. An in vitro model was developed by giving hyperglycaemic shock with 50 mM glucose to HepG2 cells which were initially adapted to normo-glucose condition of 5.5 mM. Diabetic characteristics were confirmed by insulin resistance, reactive oxygen species (ROS) production and lipogenesis. Leaf and bark of HA were collected from Western Ghats of Karnataka, throughout the year to avoid the seasonal variation in the phytochemicals. Samples were extracted with 8 solvents at two different temperatures. Extracts at sub-cytotoxic concentrations were compared on the model to check its effect on insulin resistance, ROS production and lipogenesis. The in vitro model showed insulin resistance setting at 50 mM glucose with increased ROS production and lipogenesis. Water extract of both leaf and bark reversed insulin resistance whereas acetone extract of bark reversed ROS production and lipogenesis. Activity guided fractionation needs to be done to arrive at individual or group of compounds from water and acetone extract. Top Keywords Diabetes, Medicinal plant, Holarrhena antidysenterica, Meta-analysis, in vitro model, Insulin resistance, Lipogenesis, Oxidative stress. Top |