Oxidative stress and antioxidant enzyme response in Swiss Albino Mice exposed to textile dye effluent. Kumari S. Anitha*, Pawar Anusha C., Naik S. Jithender Kumar Department of Zoology, University College for Women, Koti, Hyderabad. *Corresponding author, e-mail: anitha_shinde2001@yahoo.com
Online published on 28 June, 2017. Abstract Textile dye processing as an old age practice involves multisteps and its operations are as diverse as its product. The effluent from the textile industry contains organic and inorganic compounds with highly toxic dyes, trace elements and heavy metals. These compounds exert toxic effect on the biotic life by generating the free radicals. In order to assess the toxic nature of the textile dye effluent on the oxidative enzymes, the present study was carried out in kidney tissue of Swiss Albino Mice exposed to sub-lethal concentrations of the ¼ diluted effluent over a period of 1, 7, 14 and 22 days. The kidney tissue was isolated, perfused in cold saline and extracted for the enzymatic assays. Kidney oxidative stress byproduct of lipid, MDA (TBARS) and protein (protein carbonyl) were measured followed by the enzymatic assays of superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH). The results revealed a statistically significant (p<0.05) elevated levels of TBARS and protein carbonyl with a concomitant decline in SOD, CAT and GSH activity in all the exposed durations as against their respective control groups. The result clearly demonstrates that the compounds present in the effluent induces oxidative stress and brings an alteration in the general metabolism of mice. Top Keywords Textile dye effluent, Swiss Albino Mice, Kidney, TBARS, protein carbonyl, SOD, CAT, GSH. Top |