Correlation of VITEK-2 and conventional tests in identification of Non Fermenting Gram Negative Bacilli in a Tertiary Care Hospital Sharma Shweta1, Agarwal R K2,*, Kakati Barnali3 1Junior Resident, Dept. of Microbiology, Himalayan Institute of Medical Sciences, Swami Rama Himalayan University, Dehradun, Uttarakhand, India 2Professor & Head, Dept. of Microbiology, Himalayan Institute of Medical Sciences, Swami Rama Himalayan University, Dehradun, Uttarakhand, India 3Professor, Dept. of Microbiology, Himalayan Institute of Medical Sciences, Swami Rama Himalayan University, Dehradun, Uttarakhand, India *Corresponding Author: Email: rka1951@gmail.com
Online published on 24 September, 2018. Abstract Introduction The non-fermentative Gram negative bacilli (NFGNB) are a group of aerobic, non-spore forming, Gram negative bacilli that either do not utilize carbohydrates as a source of energy or degrade them through metabolic pathways other than the fermentation. Material and Methods The study was undertaken to identify various non-fermenters isolated from patients in our hospital, a Tertiary Care Center at Uttarakhand. 252 (14.15%) non-fermenters were isolated from 1780 clinical specimens on MacConkey and blood agar plates incubated at 35°C for 24–48 hours. Any growth that was identified as non-fermenters by VITEK-2 was subjected to identification by additional conventional tests. Results and Conclusion Patients mostly belonged to age group of 51 to 70 years and were commonly male. Sputum & ET secretion were the commonest samples. Psuedomonas aeruginosa was the commonest isolate followed by Acinetobacter calcoacetecus baumanii complex. Out of total 252 isolates, 221(87.69%) could be accurately identified upto species level by conventional tests. The conventional tests could identify 243 (96.42%) isolates upto genus level. Majority of them showed multidrug resistance. The most effective antibiotics were Tigecycline and Polymyxin B/Colistin. Top Keywords Non-fermenters, VITEK-2, Additional conventional biochemical tests. Top |