Isolation and identification of Bacillus amyloliquefaciens strains for bio-control of grapevine anthracnose Narkar Shubhangi Prakash, Sawant Indu Sanjay*, Shete Hemalata Govind1 ICAR-National Research Centre for Grapes, Manjri Farm Post, Pune-Solapur Road, Pune-412307, Maharashtra, India 1Department of Microbiology, Netaji Subhash Chandra Bose College, Nanded-431601, Maharashtra, India *E-mail: Indu.Sawant@icar.gov.in
Online published on 30 November, 2019. Abstract In India Colletotrichum gloeosporioides cause anthracnose disease of many horticultural crops, including grapes. Carbendazim resistance in the pathogen is commonly observed. Eighty-seven endophytic bacteria were isolated from mature shoots of Thompson Seedless variety located at ICAR-National Research Centre for Grapes, Pune and screened for their antagonistic activity. Ten bacteria inhibited the radial growth of carbendazim resistant C. gloeosporioides isolates by 77.59–84.59 per cent in dual culture assay. Four of these bacteria completely prevented anthracnose infections in detached leaf bio-assay and were subsequently evaluated at 104, 105, 106 CFU/ml on field grown vines. Vines treated with bacteria TS-31, TS-45 and TS-46 recorded low disease severity even at the lowest concentration of 104 CFU/ml and area under disease progress curve (AUPDC) were 456.31, 355.31, and 446.25, respectively; while in fungicide control and untreated control AUDPC was 435.19 and 756.88. In detached berries, TS-45 showed almost complete control of berry infection (2.38±4.12%), while TS-46 (20.39±3.33) and TS-31 (35.46±32.65) were less effective. These three promising bio-control bacteria were identified as Bacillus amyloliquefaciens based on morphological, biochemical and molecular characterization. Top Keywords Colletotrichum gloeosporioides, carbendazim, resistance, grape berry. Top |