(18.188.180.254)
Users online: 2514     
Ijournet
Email id
 

Indian Journal of Microbiology Research
Year : 2018, Volume : 5, Issue : 2
First page : ( 202) Last page : ( 207)
Print ISSN : 2394-546X. Online ISSN : 2394-5478.
Article DOI : 10.18231/2394-5478.2018.0043

Microbiological spectrum and antimicrobial susceptibility patterns of various isolates from endotracheal tube aspirates in a tertiary care hospital, Hyderabad, Telangana

Swati A.1,*, Yamini K.1, Rajkumar R. V.2

1Assistant Professor, Dept. of Microbiology, Kamineni Academy of Medical Sciences & Research Centre, Hyderabad, Telangana, India

2Professor & HOD, Dept. of Microbiology, Kamineni Academy of Medical Sciences & Research Centre, Hyderabad, Telangana, India

*Corresponding Author: Email: drswathi.gurajala@gmail.com

Online published on 24 September, 2018.

Abstract

Introduction

Endotracheal aspirates (ET) are relatively simple, easy to collect at the bedside and are used to evaluate the causes of lower respiratory tract infections in ventilated patients. Several studies reported multidrug resistant bacteria like Klebsiella, Pseudomonas and Acinetobacter spp, gram positive bacteria like S.aureus, S.pneumoniae, Coagulase negative staphylococcus (CONS) and fungi like Candida spp in ET cultures. Even though Quantitation of the bacteria isolated from ET aspirates has been proposed as a criterion to distinguish colonization from true infection, only few studies have been done. The main aim of the present study was to evaluate the microbiological spectrum and antimicrobial susceptibility pattern of isolates from ET aspirates.

Materials and Methods

ET aspirates were processed employing quantitative cultures. Growth of ≥105cfu/ml was considered to be significant. Organism Identification and Antimicrobial susceptibility testing was performed according to standard guidelines. The gram negative isolates were further tested for ESBL/carbapenamase production and gram positive as methicillin sensitive/methicillin resistance.

Results

Culture positivity was 67%. Acinetobacter spp was the most common isolate (46%) followed by Klebsiella spp (26%) and Pseudomonas spp (17%), E.coli (5%), S.aureus (2%), CONS (2%) and Candida (2%). Acinetobacter spp were extremely drug resistant. ESBL was produced by 38% and 64% of E. coli and Klebsiella spp. Out of the 15, 40% of the Pseudomonas spp and of 127 isolates, 63.7% of Acinetobacter spp were positive for carbapenamase production respectively. Out of the 7, 6 (86%) were methicillin resistant Staphylococcus aureus.5 (83%) of CONS (n=6) were methicillin resistant.

Conclusion

We conclude that ET microscopic examination and quantitative cultures can be supportive in earlier prediction of ventilator-associated pneumonia.

Top

Keywords

Endotracheal aspirates, Microbiological spectrum, Quantitative cultures, Multidrug resistant organisms, infection control.

Top

 
║ Site map ║ Privacy Policy ║ Copyright ║ Terms & Conditions ║ Page Rank Tool
823,984,874 visitor(s) since 30th May, 2005.
All rights reserved. Site designed and maintained by DIVA ENTERPRISES PVT. LTD..
Note: Please use Internet Explorer (6.0 or above). Some functionalities may not work in other browsers.