The Effect of Se'i (Smoked Beef) Toward the Improvement of the Bcl-2 Protein expression on Colon Cells of Balb/c strain Mice as a Carcinogenesis Indicator Adu Apris A1, Sudiana Ketut2, Martini Santi3, Mas'amah4, Husaini5 1Doctoral Program of Public Health Faculty, Universitas Airlangga Surabaya 2Faculty of Medicine, Universitas Airlangga Surabaya 3Faculty of Public Health, Universitas Airlangga Surabaya 4Faculty of Social and Political Science, Nusa Cendana University 5Public Health Study Program, Faculty of Medicine, Lambung Mangkurat University Corresponding author: Jalan Dr. Ir. H. Soekarno, No.200, Surabaya, Indonesia, email: aprisaadu606@gmail.com
Online published on 7 February, 2018. Abstract Se'i is smoked beef with added nitrite which can precipitate the Bcl-2 protein expression. The purpose of this study was to analyze the effect of se'i toward the improvement of the Bcl-2 protein expression on colon cells of Balb/c strain mice as an indicator of carcinogenesis. This research is true experimental with completely randomized design. Samples were male Balb/c mice, weighing 23.8 gram which was 36 mices, divided into 4 treatment groups P1: Mice were given se'i without nitrite with weight 8.840 mg for 28 days, P2: Mice were given se'i containing nitrite from the modern industry weighing 8.840 mg for 28 days, P3: Mice were given nitrite se'i from home industry weighing 8.840 mg for 28 days and K: Mice fed only standardize feed, and drinking water for 28 days. The examination was done by using immunohistochemical techniques to see the expression of a Bcl-2 protein. The result showed that there was a significant difference of Bcl-2 protein expression on colon cells of male Balb/c strain mice which are given se'i. The LSD test showed that there was a difference of Bcl-2 protein expression between P3, P1 and K. The new finding of this study is the provision of se'i causing the differences in Bcl-2 expression in all treatment groups. Top Keywords Se'i, nitrite, Bcl-2 expression. Top |