Preparation and Antioxidant Activity of Methanol extract of Myrmecodiarumphii Becc Pasaribu Yenni Pintauli1,*, Buyang Yorinda1, Pallitin Ivylentine Datu1, Ersam Taslim2, Nimah Yatim Lailun2 1Faculty of Teacher Training and Education, Musamus University 2Faculty of Science, Institut Teknologi Sepuluh Nopember *Corresponding author: Yenni Pintauli Pasaribu Faculty of Teacher Training and Education, Musamus University; E-mail: pasaribu@unmus.ac.id
Online published on 7 February, 2018. Abstract Ant-plant Myrmecodiarumphii Becc. usually used as traditional Papuan medicine. In the present paper, we provide for the first time the cytotoxicity and antioxidant potential of M. rumphiiBecc. This study reported cytotoxic and antioxidant activities of M. rumphiiBecc. by using maceration method for three times in every 24 hours and evaporated to obtain 77 g reddish brown concentrated. All samples were partitioned or fractionated by dichloromethane, ethyl acetate, and methanol fraction, respectively, to obtain 1 to 7 fraction. Cytotoxicity was determined using Brine Shrimp Lethality Test (BSLT) method while the antioxidant activity using 2, 2-diphenyl-2-picrylhydrazyl (DPPH) assay. The most active fraction from BSLT test is fraction 7 with LC50 is 0, 4752 μg/ml. DPPH assay shows that fraction 5 was the most potentials antioxidant with 92, 6601% radical scavenging activity. From this result indicated that the local ant-plant (Sarang Semut plant) from Merauke are high potential as a source of antioxidant. Top Keywords Ant-plant, Myrmecodiarumphii Becc, BSTL method, DPPH assay, antioxidant. Top |