Ribosomal DNA analysis of Colletotrichum lindemuthianum virulences from Himachal Pradesh (India) Padder B.A.2,*, Sharma P.N.1, Sharma O.P.1, Kapoor V.1 1Molecular Plant Pathology Laboratory, Department of Plant Pathology, CSK Himachal Pradesh Krishi Vishwavidhyala, Palampur-176 062, (HP). 2Division of Plant Pathology, SK University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar-191 121, (J&K). *E-mail: bapadder@rediffmail.com
Abstract Ribosomal DNA analysis of fifteen isolates representing 15 races of Colletotrichum lindemuthianum was restricted with 2 restriction enzymes (Msp I and Hea III) and banding pattern, obtained after digestion, analyzed for genetic diversity using 2 different molecular analytic tools (NTSYS and POPGENE). In restriction enzyme Msp I, two fragments of 340 and 100 bp were obtained, whereas two fragments of 390 and 100 bp were obtained with Hae III digestion. In some of the races additional restriction sites were noticed. Analysis with NTSYS generated a dendrogram clustering 15 races into 5 ribosomal groups (RG1 to RG5). Five haplotypes were observed in the dendrogram thereby revealing low diversity. Similar trend was observed when five polymorphic loci were analyzed with POPGENE. Total genetic diversity (Ht) varied from 1.00 to 0.39 and maximum diversity was found at locus 3 (Ht = 0.39). Top Key words Bean anthracnose, Colletotrichum lindemuthianum, genetic diversity, internal transcribed spacers, ribosomal DNA. Top |