Annals of Horticulture
Year : 2022, Volume : 15, Issue : 2
First page : ( 268) Last page : ( 272)
Print ISSN : 0974-8784. Online ISSN : 0976-4623.
Article DOI : 10.5958/0976-4623.2022.00022.6

Optimization of Culture Conditions for in Vitro Androgenesis in Papaya Varieties "Pusa Delicious" and "Tywan-786"

Sengar R.S., Rani Varsha

Department of Agricultural Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut-250110

^*Corresponding Author: Email– @gmail.com

Online Published on 22 June, 2023.

Abstract

Papaya (Carica papaya L.) is a commercially important tropical and subtropical fruit crop. Itis high in nutritional content and has medical and industrial applications. The papaya plant ispolygamous, with three sex types: male, female, and hermaphrodite. Traditional papayabreeding procedures are time demanding and necessitate the introduction of anther culture,which may be beneficial in shortening breeding cycles. The current study examines in vitroandrogenesis in papaya types “Pusa Delicious” and “Tywan-786". When anthers were cultivated on agar medium with 0.1 mg/L BA and 0.1 mg/L NAA following incubation in liquid MS mediawith 2.0% sucrose for 5 days at 30°C, the maximum embryo induction rate was reported (20.0%)in pusa delicious and (25.0%) in tywan-786.The high temperature (30°C) proved preferable forembryo induction in papaya over the somewhat lower temperature (25°C). Longer incubation ofanthers in water at these two temperatures lowered embryo induction rate. This finding willalso assist researchers build more effective anther culture techniques for furthering papayaimprovement through in vitro androgenesis.

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Keywords

Anther culture, Embryogenesis, MS media, Papaya, Plant growth regulators.

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