In vitro micropropagation of rosa damascena mill l Maheswari N Uma1, Vaishnavi K2 1Assistant Professor, Department of Microbiology, S.T.E.T Women‘s College, Sundarakkottai, Mannargudi, Tamil Nadu, India. Email id: umasamyamf@gmail.com 2Student, Department of Microbiology, S.T.E.T Women‘s College, Sundarakkottai, Mannargudi, Tamil Nadu, India. Email id: kochmicrostet@gmail.com Online published on 23 November, 2018. Abstract The application of tissue culture techniques to the regulation and commercial propagation of hybrid roses is more recent developed. Rose is one of the most popular flowering ornamentals in the world. Presently, it is a favorite ornamental for landscapes, as well as the most important commercial cut flower. The present study was planned to in vitro propagation of Rosa damascena Mill. The present study was planned to establish the efficient protocol on in vitro micro propagation of Rosa damascena Mill, and manipulating growth regulating and culture conditions. The study was demonstrated the feasibility of propagating rose in vitro. On the other hand considerable reduction in the rate of contamination (10%) with 85% survival of explants was obtained by treating the explants with sodium hypochlorite (5%) for 10 minutes after rapid rinsing with ethanol (70%) for 30 s and mercuric chloride (0.1%) for 6 minutes. The active plants were selected and subjected to shoot initiation in MS medium supplemented with growth regulators BAP, IAA and 2, 4-D. The multiple shoot induction of Rosa damascena Mill was obtained from culturing a single nodal explants on MS medium supplemented with 4.0 mg/l BA and 0.1 mg/l NAA, giving 8.27 shoots per explants in 4 weeks which could then be repeatedly sub cultured the individual newly formed shoot on the same medium. At the end of 8 weeks, a multiplication rate of 59.8cm shoot from each initial explant was obtained. Rooting was induced by sub culturing the individual new shoot on MS medium supplemented with 0.5 mg/l NAA in 4 weeks, giving 2.3 roots per shoot. After 12 weeks of growth, 90% of the plantlets obtained were established under outdoor conditions. Thus, the procedure could be used for the commercial propagation of Rosa damascena Mill. The results of our study showed that auxin IBA, IAA and NAA were the most important hormones for the form time roots and shoots. Top Keywords In Vitro, Proliferation, Initiation, Regeneration, Micro Propagation, BAP, NAA. Top |