Development and Validation of Simple UV- Spectrophotometric Method for Estimation of Diclofenac Sodium Yeola Chaitali A.1, Sonawane Vaishali N.1, Sonawane Vijayraj N.1,*, Surana Khemchand R.1, Patil Dhananjay M.2, Sonawane Deepak D.2 1Department of Pharmaceutical Chemistry, SSS’s Divine College of Pharmacy, Nampur Road, Satana, Nashik, Maharashtra, India – 423301 2Department of Pharmaceutics, SSS’s Divine College of Pharmacy, Nampur Road, Satana, Nashik, Maharashtra, India – 423301 *Corresponding Author E-mail: sonawanevijayraj@gmail.com
Online Published on 03 October, 2023. Abstract Analytical method validation is the process of proving or documenting that an analytical method produces data that can be used for what it was made for. The main goal of an analytical procedure is to get results that can be repeated and can be trusted and are good enough for the intended purpose. An effective and simple method was developed and validated for quantitative analysis of Diclofenac sodium by using UV Visible Spectroscopy. The absorption maxima (λmax) were found to be 275nm by using Distilled Water as Solvent. The λmax 275nm was used for the whole study. The linearity range was 8-16 μg/ml with a correlation coefficient (r2) of 0.9969. The method was precise and robust with a %RSD less than 2%. The solution was found to be stable up to 4 hours. The proposed method was accurate and specific as per ICH guidelines (Q2). Top Keywords UV Visible Spectroscopy, Diclofenac Sodium, Validation, ICH guidelines (Q2). Top |