Isolation and identification of compounds from the leaves extract of Hibiscus syriacus L. Rakesh Punasiya1,*, Sujit Pillai1, Janeshwer Yadav2 1GRY Institute of Pharmacy, Borawan (Khargone) 451228 2Jawaharlal Institute of Technology, Borawan (Khargone) 451228 *Corresponding Author E-mail: rakeshpunasiya@yahoo.com
Online published on 2 May, 2015. Abstract Crude extract of the leaves of Hibiscus syriacus L. was taken under phytochemical investigation. The crude methanolic extract was partitioned with different solvent system by increasing their polarities. The compounds were fractioned and isolated from n-hexane and chloroform fraction by using thin layer chrometography and column chromatography. Detection was done at 285 nm using UV spectrophotometer. The present study was designed to isolate the flavonoid from the methanolic fraction of leaves of Hibiscus syriacus L. by coloum chromatography using gradient elution method. The isolated flavonoid was characterized by spectral studies and screened for pharmacological activity on various model. Therefore the present study justifies that the isolated flavonoid exihibits significant pharmacological activity. The result indicated that the leaves of hibiscus syriacus L. may provide a rich source of flavonoids. Top Keywords Hibiscus syriacus, Polarities, chromatography, extraction. Top |