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Reverse Phase High Performance Liquid Chromatographic Method for the Determination of Ethamsylate in Bulk and Tablet Dosage Forms Nagaraju P*, Krishnachaithanya K, Srinivas VDN, Padma SVN Department of Pharmaceutical analysis, Hindu College of Pharmacy, Amaravathi Road, Guntur. India *Corresponding Author E-mail: raju_pharma35@rediffmail.com
Online published on 13 March, 2013. Abstract A simple, sensitive, precise and specific reverse phase high performance liquid chromatographic method was developed and validated for the determination of Ethamsylate in bulk and its tablet dosage forms. It was found that the excipient in the tablet dosage forms does not interfere in the quantification of active drug by proposed method. The HPLC separation was carried out by reverse phase chromatography on C18 column (4.6 x 250 mm, 5μm particle size) with a mobile phase composed of methanol: acetonitrile: acetate buffer PH 2.8 (60:30:10 v/v) in isocratic mode at a flow rate of 1 ml/min. The detection was monitored at 290 nm. The calibration curve for Ethamsylate was linear from 0.5 – 100 μg/ml. The interday and intraday precision (relative standard deviation) was found to be within the limits. The proposed method has adequate sensitivity, reproducibility and specificity for the determination of Ethamsylate in bulk and its tablet dosage forms. Top Keywords Ethamsylate, HPLC, estimation. Top | |
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