Method Development and Validation for estimation of Trihexyphenedyl Hydrochloride in Tablet Dosage Forms Patel Ashwin1,*, Shrivastava Alankar2, Jain Anurekha2, Singh GK3 1Research Associate, B.A. Research House, Opposite Pushpraj Towers, Near Judges bunglows, Ahmedabad-380054 2B.R. Nahata College of Pharmacy, Mhu-Neemuch Road, Mandsaur (M.P)-458001 3Lachoo College of Science and Technology, Shastri Nagar, sector A, Jodhpur (Rajasthan) *Corresponding Author E-mail: alankarshrivastava@gmail.com
Online published on 13 March, 2013. Abstract The development and validation of Trihexyphenidyl Hydrochloride was carried out by Ion pair extraction method. The estimation was measure at 410 nm. Against blank preparation and calibration curve was plotted. The Beer's Law was linear in concentration range of 2μg/ml to 18 μg/ml at 410 nm. The method developed was validated to determine its accuracy, precision and specificity. The recovery study was carried out by standard addition method. Top Keywords Trihexyphenidyl Hydrochloride, Ion pair extraction method, bromocresol green. Top |