Manuscript Title: Evaluation of Antimicrobial Efficacy of Three Combinations of Antibiotics Against Microorganisms in Primary Tooth Root Canal Infections-An in Situ Study Rohan Talathi1,*, Hetul Shah K2, Pallavi Pujar3, Subba Reddy4 1Senior lecturer, Department of Pedodontics and Preventive Dentistry, M.A. Rangoonwala College of Dental Sciences, Pune, India 2Professor, Department of Oral Pathology, Sri Sukhmani Dental College and Hospital, Dera Bassi, Punjab 3Senior lecturer, Department of Pedodontics and preventive dentistry, MMDC, Belgaum 4Head of Department, Department of pedodontics andpreventive dentistry, College of Dental Sciences, Davangere, Karnataka, India, 577004 *Address for Correspondence: Dr. Rohan Talathi, Senior Lecturer, Department of Pedodontics and preventive dentistry, M.A. Rangoonwala College of Dental Sciences, Pune, India, 411001, rohantalathi@gmail.com
Online published on 25 April, 2017. Abstract Background and Objective Success of endodontic therapy depends on the reduction or elimination of bacteria from root canals. Amongst the various methods to achieve this, Antibiotics are a valuable adjunct available to health professionals for the management of bacterial infections. The objective of this study was to evaluate the antimicrobial efficacy of three different combinations of antibiotics against microorganisms in gingival abscess or primary tooth root canal infections. Method 16 patients with gingival abscess or infected primary root canal were selected for the study. Two different swabs were taken using sterile paper points from the abscess or infected canals. One swab was immediately placed in test-tube containing anaerobic transport media & this swab was further used for anaerobic incubation. The other swab will be closed in container and will be used for aerobic incubation. Both the swabs will be rolled on the nutrient blood agar plate. One tablet of each antibiotic combination was placed in the petridish. The diameter of zone of inhibition in millimeter around the tablets was measured after 24 hours for aerobic incubation and 48 hours for anaerobic incubation with vernier caliper, and the data was analyzed using One-way ANOVAand Tukey's post hoc test. Results the results were expressed as mean ± SD. Metronidazole containing antibiotic combination(group A) showed significantly higher inhibition zone in both aerobic and anaerobic medium incubation when compared to ornidazole (group B) and tinidazole(group C) containing antibiotic combinations. Top Keywords Antibacterial drugs, Metronidazole, Ornidazole, Tinidazole, root canal infection, primary teeth, triple antibiotic paste. Top |