Effect of macrosized and nanoparticle manganese on serum and different organs of rats (A comparative biochemical and histological studies) Yousef Jehad M* Biochemistry Department, King Abdul Aziz University, Science Faculty for Girl's, Jeddah, Saudi Arabia *Corresponding Author: J. M. Yousef, Biochemistry Department, King Abdul Aziz university, Science Faculty for Girl's, P. O. Box 51459, Jeddah-21453, Saudi Arabia. Tel + 9661 5056 14702. Email: Jyousef@kau.edu.sa. Or Jdyousif@yahoo.com
Online published on 22 February, 2013. Abstract Manganese) Mn (is a mineral found in large quantities in both plant and animal matter. Only trace amounts of this element can be found in human tissue. However, Mn is predominantly stored in the bones, liver, kidney and pancreas. While Mn deficiency is extremely rare in humans, toxicity due to overexposure of Mn is more prevalent. Nanobiotechnology holds a huge potential in the medical sector, this field was provided by the discovery of nanoparicles metals which are used for medication. The aim of this study is to find the effect, side effect and toxicity of macrosized Mn and its nanoparticle on serum and different organs of rats (a comparative biochemical and histological studies). Seventy male Wister Albino rats, were used in the present study. They were divided into 7 groups, of 10 rats each. The first group acted as the control (drinking water). The concentration of macrosized and nanoparticle Mn is 1 mg/L of water for each. The second, third and fourth groups took, 15, 10 and 20 mg orally) macrosized Mn/(Kg of their body weight per day respectively. Fifth, sixth and seventh groups took, 15, 10 and 20 mg orally) nanoparticle Mn/(Kg of their body weight per day respectively, for 30 days. After 7, 15 and 30 days of the experiment, the rats were weighed and then anesthetized with ether and blood was collected from the eye and selected enzymatic and non enzymatic biochemical study were determined from serum. At the end of the experiment, the rats were killed by cervical decapitation and the livers, hearts and kidneys from each group were removed, and used for histological studies. From this study it was demonstrated that a decrease in nanoparticle Mn less cytotoxicity than macrosized Mn was observed at a dose of 15 mg/kg. Neutral antioxidants may offer a useful therapeutic modality to reverse the aberrant effect of nanoparticle Mn.Also, recommend the same action using different doses and for longer periods. Top Keywords Macrosized manganese, nanoparticle manganese-rats-histology. Top |