DNA Methodology: Tools, Methods, and Applications of rDNA Technology in Vegetable Crops - A Review Lone Sameena1, Narayan S.1,*, Hussain K.1, Khan F. A.2, Masoodi Khalid Z.3 1Division of Vegetable Science, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar- 190 025, J & K (India) 2Division of Basic Sciences and Humanities, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar- 190 025, J & K (India) 3Division of Plant Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar- 190 025, J & K (India) *Corresponding Author: sumatinarayan@gmail.com
Online Published on 12 September, 2023. Abstract Recombinant DNA (rDNA) methodology is a revolutionary technique in the field of molecular biology that enables scientists to manipulate and engineer DNA molecules from different sources to create novel genetic combinations. At its core, rDNA methodology involves the process of cutting and joining DNA molecules from different organisms, often from different species, to create recombinant DNA molecules. This is achieved through the use of enzymes called restriction enzymes that can cleave DNA at specific recognition sites. The resulting recombinant DNA molecules can carry genetic information from one organism and introduce it into another, thereby enabling the transfer of specific genes or genetic elements between different species. This methodology has opened up a wide range of possibilities, including the creation of genetically modified organisms (GMOs), the production of therapeutic proteins through genetic engineering, and the investigation of gene functions and regulatory mechanisms. Top Keywords Recombinant DNA methodology, Molecular biology, Restriction enzymes, GMOs. Top |