Optimization of Explant Type and Phytohormone Concentration for Micropropagation of Citrus Garg Pooja1, Sharma Nishtha1, Sharma Sonia1,*, Singh Abhinav1, Kapoor Neelesh2, Sengar Rakesh Singh2 1Department of Biotechnology and Microbiology, Meerut Institute of Engineering and Technology. Meerut 2Division of Plant Biotechnology, College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut *Corresponding Author: sonia.sharma@miet.ac.in
Online Published on 12 September, 2023. Abstract Citrus fruits are among the most consumed fruits worldwide because of their stimulating flavor and beneficial health properties. This study was designed to formulate the appropriate concentrations of plant growth regulators and activated charcoal for micropropagating lemons. In the present work, both direct and indirect organogenesis was used to successfully regenerate Citrus limon in vitro. With the help of 6-Benzylaminopurine (BAP) alone or in combination with -Naphthalene Acetic Acid (NAA), callus was activated and multiplied from leaf explants of in vitro grown seedlings on Murashige and Skoog (MS) media for indirect organogenesis. Top Keywords Citrus limon, Micropropagation, Phytohormones, Growth regulators, BAP, NAA. Top |