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Indian Horticulture Journal
Year : 2011, Volume : 1, Issue : 1
First page : ( 29) Last page : ( 31)
Print ISSN : 2249-6823.

Effectiveness of different Sterilization Treatments in Combating Contamination Problem in Papaya (Carica papaya L.)

Singh Renu*, Yadav Ram C, Yadav Neelam R

Department of Biotechnology and Molecular Biology, Chaudhary Charan Singh Haryana Agricultural University, Hisar – 125 004, Haryana, India.

*E-mail: renuboora@gmail.com

Online published on 8 May, 2013.

Abstract

Axillary bud and shoot tips from mature female papaya plants (Carica papaya L.) var. Selection 1 initially treated with different disinfectants at different concentration and time were cultured in modified MS media, each with different hormonal combination. About 43% of shoot tips explants remained free of contamination and retained regeneration while the percentage was less (34%) in case of axillary buds. Bacterial contamination of fers a severe setback in tissue culture of explants from mature female papaya plant. Therefore, in the present work different sterilization treatments to both types of explants from mature female papaya plant.

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Keywords

Carica papaya, Axillary bud, Contamination, Disinfectant, Shoot tip.

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