Antimicrobial activity of endophytes isolated from Cinnamomum tamala against human pathogens Kour Arashdeep1, Dutta Upma1,*, Gupta Moni2 1Division of Microbiology, SKUAST-Jammu, Chatha, Jammu & Kashmir-180009, India 2Division of Biochemistry, Faculty of Basic Sciences, SKUAST-Jammu, Chatha, Jammu & Kashmir-180009, India *Author for correspondence: Email: upmaduttamicro@gmail.com
Online Published on 20 January, 2024. Abstract Endophytes are the biochemical synthesizers within plants having the ability to create bioactive molecules that plants can use as a kind of defence against pathogens and some of these compounds have even been exploited in the development of valuable drugs discovery. In the present study, total 18 isolates were isolated, 6 each from bark, leaf and dry leaf of Cinnamomum tamala. The antimicrobial properties of these isolates were examined against three different human pathogens. The results revealed that out of 18 isolates, 8 isolates viz CTB-1, CTB-2, CTB-6, CTL-1, CTL-3, CTD-1, CTD-4 and CTD-6 had good antimicrobial potential against human pathogens (Klebsiella pneumonia, Pseudomonas aeruginosa and Salmonella typhi). The potential isolates were evaluated for secondary metabolite extraction using two organic solvents viz ethyl acetate and methanol, among them CTB-6 with 26.00mm, 27.00 and 28.00 zone of clearance respectively showed significant potential followed by CTD-6 with 22.00mm, 23.00mm and 24.00mm zone of clearance respectively. The potent crude bacterial extracts were studied for their minimum inhibitory concentration in additional detail. (MIC). The methanol extract of CTB-6 and the ethyl acetate extract of CTD-6 showed MIC of 20 mg/mL for Pseudomonas aeruginosa and Salmonella typhi respectively. This study concluded that the isolates CTB-6 and CTD-6 had best antimicrobial activity and further these isolates can be studied for their identification at species level and bioactive antimicrobial compounds. Top Keywords Minimum inhibitory concentration, Crude extract, Antibacterial activity, Secondary metabolites, Biochemical identification. Top |