Effect of Hippophae rhamnoides extract on Cisplatin-induced ototoxicity in Guinea Pigs Erhan Ertugrul1, Mammadov Renad2, Altuner Durdu2, Cimen Ferda Keskin3, Kurt Nezahat4, Hacimuftuoglu Fazil5, Turan Fatih1, Terzi Suat1, Celiker Metin6, Suleyman Halis2,* Department of Pharmacology, Faculty of Medicine, Erzincan University, Erzincan, Turkey 1Department of Otorhinolaryngology, Faculty of Medicine, Erzincan University, Erzincan, Turkey 2Department of Pharmacology, Faculty of Medicine, Erzincan University, Erzincan, Turkey 3Department of Pathology, Mengucek Gazi Education and Research Hospital, Erzincan, Turkey 4Department of Biochemistry, Faculty of Medicine, Ataturk University, Erzurum, Turkey 5Department of Soil Science and Plant Nutrition, Faculty of Agriculture, Ataturk University, Erzurum, Turkey 6Department of Otorhinolaryngology, Rize Education and Research Hospital, Rize, Turkey *Corresponding author E-mail: halis.suleyman@gmail.com
Online published on 16 November, 2019. Abstract No previous studies have examined the effect of Hippophae rhamnoides fruit extract (HRE), which contains both water and fat soluble vitamins, on cisplatin-induced ototoxicity. Therefore, the aim of the study was to investigate the effects of HRE on cisplatin-induced ototoxicityin Guinea pigs and toevaluatethebiochemical, gene expression and histopathological changes associated with cisplatin use. Experimental animals were divided into three groups: healthy (HG), HRE+cisplatin (HRC) and cisplatin control (CCG) groups. Biochemical, gene expression and histopathological examinations were carried on the removed inner ear tissues. In the HRC group, the oxidant parameter was lower and the anti-oxidant parameter was higher than in the CCG. These results are supported by gene expression levels and histopathological results. The use of HRE against cisplatin-induced oxidative ototoxicity may be easier, more cost effective and more beneficial than the use of vitamins alone or in combination with each other. Top Keywords Cisplatin, Ear, Gene expression, Hippophae rhamnoides, Guinea pigs. Top |