Sequencing and Polymorphism detection in growth hormone gene in Najdi Sheep and their association with milk production Afifi Mohamed1,3,6, Alkaladi Ali1, Kadasa Naif M.1,2, Saddick Salina2, Baeshen Mohammed1, Al-Farga Ammar4,*, Aqlan Faisal M.5, Almulaiky Yaaser Q.4, Alkhaled Mohammed1, Alayafi Aisha A.M.1, Ali Haytham A.3,4, Warsi Mohiuddin Khan4, Allogmani Ayed S.7, Abdelazim Aaser3,8 Department of Biochemistry, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia 1Department of Biological Sciences, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia 2Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia 3Department of Biochemistry, Faculty of Veterinary Science, Zagazig University, Egypt 4Department of Biochemistry, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia 5Department of Chemistry, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia 6University of Jeddah Center for Scientefic and Medical research, Jeddah, Saudi Arabia 7Department of Biological Sciences, Faculty of Science, University of Jeddah, khulais branch, Jeddah, Saudi Arabia 8Department of Basic Medical Sciences, College of Applied Medical Sciences, University of Bisha, Bisha, Saudi Arabia *Corresponding author's e-mail: abdalwhap.a737@gmail.com
Abstract This study was aimed to evaluate the relationship between growth hormone (GH) gene polymorphism and milk production in Najdi sheep. Two hundred Najdi ewes were used to determine the daily milk yield (DMY). The complete CDS of the Najdi sheep GH gene is1950 in length, GenBank acc. no. KU310891.1. Najdi sheep GH protein consists of 217 amino acids GenBank ALT22407.1 with conversion amino acid arginine number 121 toaminoacid lysine (R121K). A novel five SNPs were detected in Najdi sheep GH gene (G871A, A1259C, G1383A, A1486G and A1509G). A significant association between the detected SNPsgeno types and daily milk yield was found in this study. The regression analysis indicated thepositive effect of genotype and SNPs on DMY. Individuals carrying homozygote mutant alleles had the highest DMY. Consequently, these SNP may be useful indicators in the selection of ewes for milk production. Top Keywords Growth hormone gene, Milk production, Najdi sheep, SNPs. Top |