Nanoparticle based Brucella melitensis vaccine induced oxidative stress acts in synergism to immune response Kumar Amit, Gupta V.K.1, Rahal Anu2,*, Mandil Rajesh3, Verma A.K.4, Yadav S.K. Department of Veterinary Microbiology, College of Veterinary Sciences, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura-281 001, Uttar Pradesh, India 1CADRAD, Indian Veterinary Research Institute, Izatnagar-243 122, Uttar Pradesh, India 2Division of Goat Health, Central Institute for Research on Goats, Mathura-281 122, Uttar Pradesh, India 3Department of Veterinary Pharmacology, College of Veterinary Sciences, DUVASU, Mathura-281 001, Uttar Pradesh, India 4Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary Sciences, DUVASU, Mathura-281 001, Uttar Pradesh, India *Corresponding author's e-mail: rahalanu72@gmail.com
Abstract Brucella melitensis, mainly responsible for human brucellosis, is considered as biological warfare. Being intracellular in nature Brucella induces oxidative stress due to bacterial invasion in organs like spleen and lymph nodes. The present study was aimed toevaluate the specific oxidative stress responses in erythrocytesand tissues induced byintranasal inoculation of killed Brucella whole cell protein antigen coupled with sodium acrylate nanoparticle adjuvant. Glutathione mediated nonenzymic antioxidant defense system and catalase have been observed to play a critical role in intracellular antioxidant defenses in vaccinated animals and also in maintaining an excellent milieu to induce an active immune potential. The antioxidant defenses in erythrocytes, liver, kidneys, lungs and spleen of vaccinated mice also maintained excellent redox homeostasis required for quality and effective protection without inflammatory pathophysiology. Top Keywords Brucella melitensis, Immune response, Nano particle, Oxidative stress. Top |