Serum based polymerase chain reaction and enzyme linked immunosorbent assays for diagnosis of bovine brucellosis Kumar V. Naveen1,*, Bharathi M. Vijaya1, Selvaraju G.2, Porteen K.3, Vijayarani K.4 Madras Veterinary College, Tamil Nadu Veterinaryand Animal Sciences University, Chennai-600 007, Tamil Nadu, India 1Department of Veterinary Preventive Medicine, Madras Veterinary College, Chennai-600 007, Tamil Nadu, India 2Department of Veterinary Preventive Medicine, Veterinary College and Research Institute, Namakkal-637 002, Tamil Nadu, India 3Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai-600 007, Tamil Nadu, India 4Department of Animal Biotechnology, Madras Veterinary College, Chennai-600 007, Tamil Nadu, India *Corresponding author's e-mail: naviviswanathan300@gmail.com
Abstract Brucellosis is one of the economically important diseases in India and diagnosis of brucellosis using single test is cumbersome due to variation in sensitivity and specificity among the different test. The present study was aimed to assess the suitability of serum as clinical specimen in molecular diagnosis and evaluate the serology and molecular assays as in diagnosis of bovine brucellosis. Atotal of 821 bovine serasamples were subjected to indirect Enzyme Linked Immunosorbent Assay (i-ELISA) and serum based Polymerase Chain Reaction (PCR) assay. On serology 6.70 per cent positivity of brucellosis were reported and on PCR assay, 47 and 29 sera samples were positive for bcsp 31 genus specific and IS711 species specific PCR assay respectively with per cent positivity of 5.72 and 3.53. In comparison between serology and molecular test, 44 samples were positive for both assays and 11 and 3 samples were positive for serology and molecular assays individually. This study suggests that serum sample can be utilised as the choice of clinical specimen for both PCR assayand i-ELISA will be a future choice for the diagnosis of bovine brucellosis. Top Keywords Bovine, Brucellosis, i-ELISA, Serum, Serum PCR. Top |