Characterisation of KiSS1R gene and Non genetic factors affecting reproductive traits in Indian goats Sahoo Siddhant Sekhar1, Mishra Chinmoy, Mohanty Stuti Tanaya, Kaushik Rakesh, Rout P.K., Singh M.K., Dass Gopal, Sethy Kamdev, Behera Kumaresh, Dige M.S.* ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura-281 122, Uttar Pradesh, India 1Division of Animal Genetics and Breeding, ICAR-CIRG, Makhdoom, Mathura-281 122, Uttar Pradesh, India *Corresponding author's e-mail: maheshdige@gmail.com
Online published on 1 August, 2019. Abstract This study was undertaken to explore the genetic polymorphism in the KiSS1R (GPR54) gene from 80 Black Bengal, 50 Ganjam and 20 Raighar goat. Each of the sampled goats was recorded for its reproductive traits. The genomic DNA was isolated from the collected blood samples. The target 3' UTR comprising of 246 bp fragment of KiSS1R gene was successfully amplified using the specific primer. Amplified samples were subjected for HRM analysis followed by sequencing. The nucleotide sequence alignment with the retrieved DNA sequence from NCBI BLAST confirmed absence of polymorphic pattern in KiSS1R gene in 3' UTR. However, in the studied populations breed had significant effect on littersize, kidding interval and age at sexual maturity. It was found that age at sexual maturity and kidding interval were the highest in Ganjam goat population as compared to Raighar and Black Bengal goat population. Litter size was found highest in Black Bengal goat. Top Keywords BLAST, Gene, Goat, KiSS1R, Reproduction, SNP. Top |