Effect of antioxidant ascorbic acid on in vitro maturation of Caprine oocytes under normal and elevated temperatures Khanday S.B., Ahmed J.A.*, Nashiruddullah N.1, Sharma U.2, Chakraborty D.3 Division of Veterinary Physiology and Biochemistry Faculty of Veterinary Science and Animal Husbandry, Sher-e-Kashmir University of Animal Sciences and Technology. R.S. Pura-181 102, Jammu and Kashmir, India 1Division of Veterinary Pathology, R.S. Pura-181 102, Jammu and Kashmir, India 2Division of Veterinary Gynaecology and Obstetrics, R.S. Pura-181 102, Jammu and Kashmir, India 3Division of Animal Genetics and Breeding, R.S. Pura-181 102, Jammu and Kashmir, India *Corresponding author's e-mail: jafrinahmed@rediffmail.com
Online published on 30 August, 2019. Abstract The aim of the present study was to assess the effect of ascorbic acid on in vitro maturation of caprine oocytes under normal and elevated temperatures. Goat ovaries were collected at slaughter and both A and B grade cumulus-oocyte-complexes (COCs) were aspirated out and were matured in vitro under normal (38.5°C) and elevated temperatures (41°C). On the basis of cumulus expansion and nuclear maturation, the maturation competencewere compared with and without ascorbic acid supplementation (100 μM). Heat stress significantly (P≤ 0.01) reduced cumulus expansion, maturation rate and lowered metaphase stage II of nuclear maturation. Ascorbic acid improved developmental competence of oocytes during heat stress (41°C) and ascorbic acid supplemented COCs demonstrated significantly (P≤ 0.05) higher maturation rates when compared to non-supplemented groups. Top Keywords Ascorbic acid, Goat oocytes, Heat stress, In vitro maturation. Top |