Molecular detection and phylogenetic analysis of avian reticuloendotheliosis virus from formalin fixed tissue by PCR in fancy chicken Ponnusamy P.*, Reetha T. Lurthu, Sasikala M., Ronald B.S.M., Selvaraj J., Puvarajan B., Manickam R. Department of Veterinary Microbiology, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Orathanadu-614 625, Tamil Nadu, India *Corresponding author's e-mail and address: ponsvhl@gmail.com; Department of Veterinary Microbiology, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Orathanadu-614 625, Tamil Nadu, India.
Online published on 15 October, 2019. Abstract The present study was carried out to identify avian neoplastic viruses in the formalin fixed tissues of fancy chicken. The tissue samples collected during necropsy were examined by histopathology. It showed lymphoid and reticular cell infiltration in kidney, liver and lungs. For identification and differentiation of avian neoplastic viruses, PCR was performed using primer sets specific for Mareks disease virus, avian leukosis complex and reticuloendotheliosis virus. It was found that tumors were REV originated. Further confirmation, purified PCR product was subjected to sequencing. It showed 99% homology with other REV isolates available in the NCBI database. The present communication describes infection of a fancy chicken with REV on the basis of histopathological findings as well as molecular methods. Top Keywords Fancy chicken, PCR, Phylogenetic analysis, Reticuloendotheliosis virus. Top |