Optimization and identification of lactic acid bacteria with higher mannitol production potential Saleena Lejaniya Abdul Kalam1,**, Chandran Deepak2,*, Geetha R.3, Radha R.3, Sathian C.T.3, Kumar Manoj4, Sureshkumar R.5, Marthandan V.5, Radha6 1Department of Food Science and Nutrition, Faculty of Applied Sciences, 1, UCSI Heights, Jalun Pancak Menara Gading, UCSI University, Taman Connaught, 56000, Cheras, Willayah Persekutuan, Kuala Lampur, Malaysia 2Department of Veterinary Sciences and Animal Husbandry, Amrita School of Agricultural Sciences, Amrita Vishwa Vidyapeetham University, Coimbatore-642 109, Tamil Nadu, India 3Department of Dairy Science, College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680 651, Kerala, India 4Division of Chemical and Biochemical Processing, ICAR-Central Institute for Research on Cotton Technology, Mumbai-400 019, Maharashtra, India 5Amrita School of Agricultural Sciences, Amrita Vishwa Vidyapeetham University, Coimbatore-642 109, Tamil Nadu, India 6School of Biological and Environmental Sciences, Shoolini University of Biotechnology and Management Sciences, Solan-173 229, Himachal Pradesh, India *Corresponding Author: Deepak Chandran, Department of Veterinary Sciences and Animal Husbandry, Amrita School of Agricultural Sciences, Amrita Vishwa Vidyapeetham University, Coimbatore-642 109, Tamil Nadu, India, Email: c_deepak@cb.amrita.edu
**Lejaniya Abdul Kalam Saleena, Department of Food Science and Nutrition, Faculty of Applied Sciences, 1, UCSI Heights, Jalun Pancak Menara Gading, UCSI University, Taman Connaught, 56000, Cheras, Willayah Persekutuan, Kuala Lampur, Malaysia, lejaniya@gmail.com
Online Published on 5 January, 2024. Abstract Background Lactic acid bacteria (LAB) is considered as food-grade microorganism with generally recognized as safe (GRAS) status. Both hetero-fermentative and homo-fermentative LAB have the ability to produce mannitol as metabolic end product in normal fermentation. Methods Ten LAB isolates selected were detected for its mannitol production potential using colorimetric assay in preliminary study. Later selected four isolates of LAB with better mannitol production were further optimized at different culture conditions. A total of 540 mannitol combinations were obtained after optimization. All the observations were statistically analyzed using response surface methodology. Biochemical and molecular assays were carried out to identify the isolates. Result The isolate L8 produced mean mannitol content of 1.635 g/L, 0.345967 cell densities, at pH 7.0 and temperature 42°C with agitation of 100 rpm was selected with optimum response surface optimization because of its higher mannitol production. Biochemical and molecular assays identified higher mannitol producers, L4 as Enterococcus faecium strain Gr17, L6 as Lactobacillus rhamnosus strain 6870, L8 as Leuconostoc pseudomesenteroides culture IMAU: 11666 and L9 as Lactobacillus plantarum subsp. plantarum strain NMB8. Top Keywords Biochemical and molecular assay, Colorimetic assay, LAB, Mannitol, Sequencing. Top |