Genetic diversity of crossbred cattle using cytochrome oxidase subunit I (COI) gene in South Sulawesi, Indonesia Utami Sri1,2, Ali Jamil3, Nidom Reviany V.4,5, Nasir Muhlis6, Prayitno Haris6, Dhani Prakoso4, Ansori Arif N.M.4, Indrasari Setyarina4,7, Nidom Chairul A.2,4,5,7,* 1Agriculture Quarantine Major Service of Surabaya, Ministry of Agriculture, Surabaya, Indonesia 2Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia 3Directorate General of Facilities and Infrastucture, Ministry of Agriculture, Jakarta, Indonesia 4Professor Nidom Foundation, Surabaya, Indonesia 5Riset AIRC Indonesia, Surabaya, Indonesia 6Agriculture Quarantine of Jayapura, Jayapura, Indonesia 7PNF-Animal Diagnostic-Laboratory, Surabaya, Indonesia *Corresponding Author: Chairul A. Nidom, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia, Email: nidomca@fkh.unair.ac.id
Online Published on 8 February, 2024. Abstract Background The genetic diversity information of local cattle and crossbred in South Sulawesi, Indonesia is essential for the establishment of conservation and breeding strategies. This research targeted to evaluate the phylogenetic relationship and genetic diversity of local and crossbred (CB) cattle. Methods The present research organized at the Animal Laboratory, Professor Nidom Foundation, Surabaya, Indonesia from February to October 2021. The genome was isolated from 20 whole blood samples from seven purebreed belongs to Singosari National Artificial Insemination Center Malang, Indonesia, four crossbred in Gowa and nine crossbred in Sidrap South Sulawesi, Indonesia. Partial sequences of mtDNA cytochrome oxidase subunit I (COI) gene were amplified employing the polymerase chain reaction technique. Result This study revealed that a lot of single nucleotide polymorphism (SNP) found in local cattle (Bali and Peranakan Ongole) and CB cattle in Gowa and Sidrap indicated various genetic diversity can be used as a marker candidate and potential to be developed into future research knowing the correlation of the SNP with the growth rate of CB. Thus, this information will be helpful for further strategy in breeding development program. Top Keywords COI, Crossbreed, Genetic diversity, Multidimensional scaling, Phylogenetics. Top |