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Indian Journal of Entomology
Year : 2019, Volume : 81, Issue : 1
First page : ( 182) Last page : ( 190)
Print ISSN : 0367-8288. Online ISSN : 0974-8172.
Article DOI : 10.5958/0974-8172.2019.00005.1

Analysis of brown planthopper Nilaparvata lugens Feeding induced volatiles in rice cultivars

Navyashree R.K.1, Soundararajan R.P.2, Subbarayalu Mohankumar1,*

1Department of Plant Biotechnology, Centre for Plant Molecular Biology & Biotechnology, Tamil Nadu Agricultural University, Coimbatore, 641003

2Department of Rice, Tamil Nadu Agricultural University, Coimbatore, 641003

*Corresponding author Email: smktnau@gmail.com

Online published on 30 April, 2019.

Abstract

The brown plant hopper (BPH) is an economically important insect pest of rice. Herbivore induced plant volatiles (HIPVs) have greater role in plant defense as repellents or feeding inhibitors or cues for natural enemies. In this study, volatiles induced after BPH infestation from susceptible (TN1) and resistant (Ptb33) rice varieties were identified using Gas Chromatography Mass Spectrophotometer (GCMS) and NIST MS 2 library. Ptb33 infested samples had defensive volatiles like stigmasterol, β-sitosterol, hexadecenoic acid, benzothiazole, phytol, nonanal with highest peak area %. In infested samples of TN1, only a few defense related volatiles like α-copaene were found. Eleven and sixteen overlapping volatiles were identified in infested and uninfested samples of TN1 and Ptb33, respectively. In addition to the host plant resistance in Ptb33 to BPH, induced volatiles provide indirect defense.

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Keywords

Brown planthopper, resistance, induced volatiles, GC-MS, AMDIS, MZmine2.

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