Insecticidal property of Citrus sinensis fruit waste against papaya mealybug Paracoccus marginatus Gowtham M., Shanthi M.#, Kumar S. Vellai*, Premalatha K. Department of Entomology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai, 625104 *Department of Biotechnology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai, 625104 #Email: cshanthiento07@gmail.com (corresponding author)
Online published on 24 October, 2019. Abstract Laboratory experiments were conducted during August to March, 2018–2019, to evaluate the insecticidal property of Citrus sinensis (L.) Osbeck fruit waste viz., peel and seed against the papaya mealybug Paracoccus marginatus (Williams and Granara de Willink). Five solvents, viz., hexane, ethyl acetate, methanol, diethyl ether and dichloromethane were used to extract the bioactive compounds. The efficacy of these extracts was evaluated against P. marginatus at 10000 ppm for their oral or contact toxicity, through leaf contamination and dry film method, respectively. The methanol extract was observed to exert maximum mortality of P. marginatus in leaf contamination method (88.89% and 86.11%, respectively) and also dry film method (88.33%, 78.06%, respectively) after 72 hr after treatment. The chemical profile of the methanol extract of seed and peel analysed with GC-MS was also done. Top Keywords Citrus sinensis, peel and seed, methanol extract, Paracoccus marginatus, oral and contact toxicity, leaf contamination, dry film, GC-MS analysis. Top |