Assessment of Semen Bio Markers in Vaginal Swabs in Sexual Assaults Agnihotri Ashok Kumar1,*, Mishra Anshu2, Kesarvani Ankit3, Singh Mona3, Singh Gunbir4 1Lecturer, Department of Forensic Medicine & Toxicology, Government Medical College, Kannauj, Uttar Pradesh, India 2Professor, Department of OBS & Gynecology, GSVM Medical College, Kanpur, Uttar Pradesh, India 3Junior Resident, Department of Forensic Medicine & Toxicology, Government Medical College, Kannauj, Uttar Pradesh, India 4MBBS, Government Medical College, Amritsar, Bir Hospital, Ludhiana, Punjab, India *Corresponding author, Dr. Ashok Kumar Agnihotri, Lecturer, Department of Forensic Medicine & Toxicology, Government Medical College, Kannauj, UP, India, E-mail: drashokagnihotri593@gmail.com
Online published on 7 May, 2019. Abstract Introduction Semen is considered a diagnostic fluid in forensic science which helps in diagnosis of sexual assault. The present study was conducted to determine semen biomarkers in vaginal fluid in suspected rate victims. Materials & Method The present study found 20 rape victim out of 94 women. In all, vaginal swabs were obtained. In this, Semen cytology (SC), prostate-specific antigen (PSA) and Y chromosome DNA was detected with polymerase chain reaction. Results Maximum victims were seen in age group 10–20 years (32) followed by 20–30 years (29), 30–40 years (28), 40–50 years (4) and >50 years (1). It was found that 20 cases were found to be positive of rape. Sensitivity of SC was found as 70%, PAS as 80% and PCR as 100%. Specificity of SC was 88%, PSA 92% and PCR as 96%. Table III shows various primers used in the study. Conclusion Semen biomarkers such as SC, PSA and Yc-DNA are widely used and accepted methods for detection of offense. They have high specificity and sensitivity rate. Top Keywords Rape, Semen biomarkers, Semen cytology. Top |