Comparative Study of Age Estimation by Cemental Annulations by Polarizing and Light Microscopic Methods Using Digital Method Adobe Photoshop 7 Version Bommanavar Sushma1,*, Joshi Shubha2, Mashalkar Vaishali3, Baad Rajendra4, Vibhute Nupura5, Belgaumi Uzma6, Kadashetti Vidya7 1Senior Lecturer, Department of Oral Pathology & Microbiology, School of Dental Sciences KIMS “Deemed to be university”, Karad 2Associate Professor, Department of Prosthodontics and Crown & Bridge, School of Dental Sciences KIMS “Deemed to be university”, Karad 3Senior Lecturer, Department of Periodontology, School of Dental Sciences KIMS “Deemed to be university”, Karad 4HOD, Department of Oral Pathology & Microbiology, School of Dental Sciences KIMS “Deemed to be university”, Karad 5Associate Professor, Department of Oral Pathology & Microbiology, School of Dental Sciences, KIMS “Deemed to be university”, Karad 6Associate Professor, Department of Oral Pathology & Microbiology, School of Dental Sciences, KIMS “Deemed to be university”, Karad 7Senior Lecturer, Department of Oral Pathology & Microbiology, School of Dental Sciences, KIMS “Deemed to be university”, Karad *Corresponding author: Dr. Sushma. Bommanavar (MDS-Senior Lecturer) Department of Oral Pathology & Microbiology, School of Dental Sciences, KIMS “Deemed to be university”, Karad, Phone: -+91 9673695306, Email: drsushopath@gmail.com
Online published on 7 May, 2019. Abstract Background Cementum formed as continuous process throughout life consists of alternate light and dark bands known as incremental lines of Salter. Using different types of microscopy in forensic odontology, the number of these incremental lines is used to estimate the age of an individual. Aim and Objectives To examine the correlation between age and tooth cemental annulations using light and polarizing microscopy with Adobe Photoshop Software 7 Version and to compare both the methods to ascertain the most reliable method of studying cementum. Materials and Method A cross sectional descriptive, double blind study was conducted during the period of March 2016 to March 2017. 60 extracted monoradicular teeth from individuals in the age group of 20–80 years (Females=42, Males =18) were randomly collected and longitudinal ground sections of the teeth were prepared, mounted and examined under light and polarizing microscope. Digital images were captured, magnified & cemental lines were counted using the Adobe Photoshop 7 Version by two anonymous oral pathologists who had no idea of the research study. Statistical analysis was done using SPSS 10 version. Results There was a strong positive correlation between the estimated age and calculated age when light microscopy was used (r=0.725, p =0.00). The correlation was less with polarizing microscopy (r=0.697, p=0.00) and the cemental annulations were better appreciated and easily counted using the Adobe Photoshop Software 7 Version. Conclusion Our findings suggest that Adobe Photoshop Software 7 Version provides better visibility of counting the cemental lines and therefore can estimate accurate age of an individual in forensic Odontology. Top Keywords Tooth cemental annulations, Age estimation, Adobe photoshop 7 version, Polarizing microscopy, Light microscopy. Top |