Metagenomics Shows Ubiquitous Nature of Red Complex Bacteria, Favoring New Classification of Periodontitis Balaji Anitha1, Mahalakshmi K.2,*, Valiathan Mohan3, Prasanth B Krishna4 1Professor, Department of Periodontics, Sree Balaji Dental College, BIHER, Chennai 2Professor & HOD, Department of Microbiology, Sree Balaji Dental College, BIHER, Chennai 3Professor, Department of Periodontics, Sree Balaji Dental College, BIHER, Chennai 4Assistant Professor, Department of Epidemiology, Sree Balaji Dental College, BIHER, Chennai *Corresponding Author: Dr. K. Mahalaksmi, email id-kmagvenkat@gmail.com
Online published on 7 May, 2019. Abstract Background Classification of periodontal lesions has always been a difficult and dynamic issue, due to constant addition of knowledge that enriches our understanding of periodontal pathophysiology. With current evidence from metagenomics, it is necessary to find the association of oral microbiota to periodontal lesions. Materials and Method Current study is cross-sectional, and samples (subgingival plaque) were collected from 6 individuals classified in to 2 groups., Viz. Aggressive periodontitis and Chronic periodontitis. The study excluded patients who underwent Antibiotic therapy within the past 3 months, Pregnant women, systemically unhealthy individuals, tobacco(in any form) user and alcoholics. Metagenomic analysis was conducted on subgingival plaque and results were tabulated with respect to red complex bacteria. Results It was observed that, red complex bacteria have not differed significantly between the chronic and aggressive periodontitis patients. Conclusion The new classification of periodontal lesions, 2017 is justified by classifying all microbial diseases in to one group as there is no evidence in metagenomics to separate them. But, the difference in clinical manifestation has to be further investigated to arrive at better treatment modalities. Top Keywords Metagenomics, Red Complex, New classification. Top |