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Indian Journal of Forensic Medicine & Toxicology
Year : 2019, Volume : 13, Issue : 3
First page : ( 335) Last page : ( 340)
Print ISSN : 0973-9122. Online ISSN : 0973-9130.
Article DOI : 10.5958/0973-9130.2019.00219.6

Isolation and Antibiotic Susceptibility of Anaerobic Bacteria of Periodontitis in Diabetic and Non-Diabetic Patients in Basrah Province, South of Iraq

Al-Farhan Salah Razzaq1, Al-Mussawi Abdulameer Abdullah1,*, AL-Abdulla Amani Abd AL-Ridha1

1College of Science, University of Basrah, Basrah, Iraq

*Corresponding author: Abdulameer Abdullah Al-Mussawi. College of Science, University of Basrah, Basrah, Iraq, E-mail: dr_ameer2006@yahoo.com

Online published on 17 July, 2019.

Abstract

Periodontitis considered as polymicrobial disease since that the primary etiologic agents is sub gingival plaque bacteria, while the specific pathogens interaction is more relevant in the progress of the disease than are individual's species. Samples resulted in isolation of 66 bacterial isolates include 38 isolates were diagnosed with bacteriological methods belong to 10 species of 6 genera confirmed by Vitek 2 Compact System, Anaerococcus prevotii, Lactobacillus fermentum Biofidobacterium spp, Peptophillus asaccharolytiucs, Actinomyces israelii, Actinomyces odontolyticus, Lactobacillus plantarum, Lactobacillus parcasei and Actinomyces naeslundii. The Epsilometer-test reveal that the species Anaerococcus prevotii, Lactobacillus plantarum and Actinomyces israelii showed resistance to Tetracyclin, while Anaerococcus prevotii showed resistance to Clarithromycin, Nalidixic Acid and Tetracyclin. The species Anaerococcus prevotii was sensitive to Ciproflaxacin, while Peptophillus asaccharolytiucs was sensitive to tetracycline and resistant to Nalidixic Acid. Samples were collected during the period between May 2016 and April 2017. GCF was collected by absorbent paper points from a total 73 patients suffering from periodontitis comprised 41(56.16%) specimens of diabetic patients and 32(43.83%) specimens of non-diabetic patients. No significant differences were observed (p>0.05), including 32 male represent (43.38%) and 41 female represent (56.16%) with no significant differences in the percentages of isolation between the sexes (p>0.05).

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Keywords

Anaerobic Bacteria, Periodontitis, Diabetic, Vitek, Antibiotic susceptibility.

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