Study of Phenotyping and Genotyping Characterization of Methicillin Resistant Staphylococcus Aureus Isolates Al-Jumaily Sarah Fawzi S.1,*, Al-Taai Hadi R. Rasheed1, Al-Dulaimi Abbas A.1 1Biology Department, College of Pure Science, Diyala University, Iraq *Corresponding author: Sarah Fawzi S. Al-Jumaily, E-mail: sara.fawzi8@gmail.com
Online published on 27 November, 2019. Abstract The current study included collection of one hundred and ten samples from patients in Baquba Teaching Hospital, in Diyala during the period from June 2018 till the end of August 2018. The specimens included swab from wounds, burns, abscesses. All isolates were diagnosed depending on macroscopic, biochemical tests and Vitek 2 it was found that 80 (72.7%) isolates were identified as Staphylococcus aureus and 50 (62.5%) of isolates were Methicillin resistant Staphylococcus aureus (MRSA). In this study, MRSA isolates were showed high resistance against five types of antibiotics. It was as follows; Oxacillin 100%, Tetracycline 100%, Amikacin 88%, Vancomycin 9.7%, Gentamycin 76%. MIC of Ag NPs and TiO2 NPs is ranged between (325–5200 μg/ml) and (40.6–2600 μg/ml) respectively on MRSA isolates. The technique used in this study to determine genetic diversity of MRSA was MLST (Multi locus sequence typing). Seven housekeeping genes primers: arc (Carbamate kinase), aro (Shikimate dehydrogenase), glp (Glycerol kinase), gmk (Guanylate kinase), pta (Phosphate acetyltransferase), tpi (Triosephosphate isomerase), yqi (Acetyle coenzyme A acetyltransferase) were taken from website Institute Pasteur. Five isolates have been selected for PCR amplification reaction. Nucleotide variances were seen and specific alleles for each locus were designated. The allele profile for each isolate were designated. The allele profile for each isolate was then used to determine sequence type (ST). To the best of our knowledge, this was the first study that involved an MLST analysis of clinical S. aureus isolates from hospital in Diyala, Iraq. Top Keywords MRSA, antibiotics, biofilm, nanoparticles, MLST. Top |