Indian Journal of Forensic Medicine & Toxicology
Year : 2019, Volume : 13, Issue : 4
First page : ( 1036) Last page : ( 1042)
Print ISSN : 0973-9122. Online ISSN : 0973-9130.
Article DOI : 10.5958/0973-9130.2019.00436.5

The Role of PLEKHM1 in Salmonella enterica sv. Typhimurium infection

Radhi Ohood Aqeed

Sub Department of Basic science, College of Nursing, University of Kufa, Iraq

Online published on 27 November, 2019.

Abstract

Background

Recent evidence has found that PLEKHM1 plays a role for Salmonella enterica sv. Typhimurium infection. Salmonella enterica sv. Typhimurium utilises PLEKHM1 to tether phagolysosome membranes to the SCV. This membrane remodelling provided a protective niche for proliferation in primary cells, and in tissues Aims: Here, we aimed to target PLEKHM1 in HEK293A cells for the prevention of Salmonella enterica sv. Typhimurium infection. Targeting the PLEKHM1 protein could possibly inhibit cell killing by Salmonella enterica sv. Typhimurium, which could help infections. That maybe has medical applications in the future. CRISPR technique was used to knockout PLEKHM1 in HEK293A cell. Biochemical assay (western blot) was used to determine this knockout. Biological assay (Microscopy): Imaging Experiments for confocal microscopy was also used to investigate the invasion of bacteria inside cells. The results found that CRISPR targeting of PLEKHM1 in HEK293A gave clear resistance to the cells following infection by Salmonella enterica sv. Typhimurium indicated the role of PLEKHM1 in Salmonella enterica sv. Typhimurium infection. The PLEKHM1 has role in Salmonella enterica sv. Typhimurium induces cells death. Therefore, pharmacological inhibition of the PLEKHM1 or its components can be explored as future work to modulate infection by Salmonella enterica sv. Typhimurium.

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Keywords

Salmonella enterica sv. Typhimurium, PLEKHM1, HEK293A.

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