Histological and Flourescent Microscope Studies for Evaluation of Carbon Accumulation in Lymphatic tissues in Birds within Polluted Areas (Anasplatyrhynchos) Aleiqabi Rafel F.J1, Al-Hamadiny Shahad Q.1, Al-Zamily Khalied Y.Z.2, Al-awsy Melad1, Faraj Safaa A.3, Al-Deresawi Tayseer Shamran4, Dilfi Saja Hussain1 1Biology Department, College of Sciene, University of Wasit, Iraq 2Pathological Analysis Department, Kut Technical Institute, Middle Technical University 3College of Medicine, University of Wasit, Iraq 4Biology Department, College of Education for pure Sciene, University of Wasit, Iraq Online published on 27 November, 2019. Abstract In order to follow up the deposition of carbon in the lymphatic tissues of birds, 18 birds of the ducks were collected. Divided into three groups of (6) birds per group. All the lymphatic tissues of the cecal tonsils and the lymph node in the lung of the birds of polluted areas contain the carbon aggregations in a variable manner. Carbon aggregations were slightly higher in the lymphatic tissue collected from brick factories polluted areas than those collected from oil fields polluted area. The effect of carbon aggregations on all lymphatic tissue cells was evident in this study, which caused the death of these cells (programmed death), which is clearly demonstrated by using AO/EB double stain microscope technique, where normal cells appeared green, and other cells appeared in yellow color which indicates early apoptosis, while other cells appear orange as an indicator of the late stage of cell death. Top Keywords Carbon, Acridine orange/Eithiedium bromide, Flourescent microscope, cecal tonsils, lung lymphoid nodes. Top |