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Indian Journal of Forensic Medicine & Toxicology
Year : 2019, Volume : 13, Issue : 4
First page : ( 1780) Last page : ( 1786)
Print ISSN : 0973-9122. Online ISSN : 0973-9130.
Article DOI : 10.5958/0973-9130.2019.00569.3

Peanut Sucrose for Modifications Medium on Growing of Candida Albicans and Tinea Versicolor

Sasongkowati Retno1,*, Christyaningsih Juliana2, Suliati1

1Health Analyst Department, Health Polytechnic of Surabaya

2Nutrition Department, Health Polytechnic of Surabaya

*Corresponding author: Retno Sasongkowati, E-mail: retnosasongkowati@yahoo.co.id, Address: Pucang Jajar Tengah Street-56, Surabaya, Indonesia

Online published on 27 November, 2019.

Abstract

The potency of local peanut can be utilized as fungi growth medium to substitute Potato Dextrose Agar media (PDA). Peanuts are widely found in East Java and there are 7 varieties, namely Takar-1, Takar-2, Tuban, Jerapah, Talam-1, Hypoma-2, and Bima. This study aimed to make modified media from nuts for growth of Candida albicans, and Tinea versicolor. The type of this research was exploratory design. Peanut Sucrose Agar (PSA) medium was made from 8 local peanut varieties, sucrose, agar, chloramphenicol. As a gold standard media used PDA and Sabaroud Dextrose Agar (SDA). The mediums were planted with Candida albicans, and Tinea versicolor, to analyze colony diameter, morphological features and storability PSA media. The largest diameter of Candida albicans colonies and the highest number of Tinea versicolor colonies were obtained in PSA from Talam-1 variety when compared with PDA and SDA media. There was no difference in morphological of Candida albicans features and Tinea versicolor on PSA, PDA and SDA media. Storability of PSA, PDA and SDA media for 1 month at 4°C does not cause macroscopic media changes. Local peanut varieties Talam-1 was the best variety as the base material for the manufacture of PSA media for the growth of Candida albicans and Tinea versicolor.

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Keywords

Candida albicans, Tinea versicolor, Peanut sucrose agar.

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