Molecular characterization and relationship among wild and partially cultivated Rosa species Veluru Aparna*, Bhat K.V.2, Janakiram Tolety3, Prasad K. V.1, Raju D.V.S1, Namita, Panwar Sapna, Singh K. P. Division of Floriculture and Landscaping, ICAR-Indian Agricultural Research Institute, New Delhi- 110012, NCT DelhiIndia 1ICAR- Directorate of Floriculture Research, Pune, 411 005, Maharashtra, India 2ICAR- National Bureau of Plant Genetic Resources, Pusa, New Delhi, 110 012, India 3Dr Y.S.R.H.A.U, Venkataramannagudem, 534 101, West Godavari, Andhra Pradesh *Corresponding author: aparna.cpcri@gmail.com
Online Published on 23 December, 2022. Abstract Knowledge of population structure and genetic relationships within and among wild rose (Rosa spp.) genotypes is important to enhance its use in breeding and conservation. In the present study, we assessed the diversity among 21 wild roses belonging to 7 sections of subgenera Rosa, using morphological (14) and molecular markers (48). The neighbor-joining (NJ) tree clustered the species into two major and four minor clusters based on morphological similarity. Of the used SSRs, 31 SSRs were found to be polymorphic within selected genotypes. The matching co-efficient value calculated for each pair of species revealed the highest (0.91) and lowest (0.386) genetic similarity between species Rosa brunonii (Synstyle) -R. indica major (Indicae) and R. multiflora (Synstyle) -R. slancensis (Caninae). The phylogenetic chart obtained using molecular data clustered the genotypes in two major clusters.R. bourboniana exhibited a distant relationship with the remaining 20 genotypes and clustered separately. All the species from Gallicanae and most of the Indicae section species were clustered together and showed genetic similarities with each other. Significant variability was noticed among cultivated R. damascene selections. A local fragrant loose flower variant namely ‘Kakinada Red’ which is cultivated widely in southern peninsular India under tropical climate showed genetic nearness with Damascena cv. Ranisahiba. The Bayesian analysis conducted among genotypes showed three distinct genetic groups (AK=3). Concerning the genetic variability among different sections, highest and lowest variability values were observed with Synstyle and Pimpinellifoliae, respectively. Top Keywords Rosa species, Morphological markers, SSR markers, Characterization, Genetic diversity. Top |