Aonla pomace as substrate for endoglucanase production using Trichoderma harzianum Kumar Devendra1, Garg Neelima1,*, Muthukumar M.2, Yadav Kaushlesh K.1 1Division of Post Harvest Management, Central Institute for Subtropical Horticulture, Rehmankhera P.O.Kakori, Lucknow – 227107 2Division of Crop Improvement and Biotechnology, Central Institute for Subtropical Horticulture, Rehmankhera, P.O.Kakori, Lucknow – 227107 *Corresponding author– neelimagargg@rediffmail.com
Online published on 4 December, 2019. Abstract Submerged fermentative (SmF) production of endoglucanase by a cellulolytic fungus Trichoderma harzianum any isolet number using aonla (Emblica officinalis Gaertn.) pomace as a substrate was standardized. Optimum production of the enzyme (0.57 ± 0.05 U/ml) was obtained at pH 4.0 and 30 ºC temperature using substrate concentration of 1.5% (w/v) supplemented with ammonium sulphate (1.5% w/w of substrate) as nitrogen source on 3rd day of fermentation. The soluble extracellular protein was extracted and partially purified by acetone to an extent of 2.395 fold. Protein profiling on 12% Sodium dodecylsulfate-Polyacrylamide gel electrophoresis (SDS-PAGE) revealed three bands with molecular size Corresponding to 86, 68 and 46 kDa, suggesting the isomeric forms of endoglucanase. The optimum endoglucanase activity in partially purified sample was achieved at pH 6.0 and 30 ºC temperature. The Michaelis-Menten constant (KM) and Vmax were found to be 13.89 mg/ml, and 0.215 μmole/min/mg, respectively. Top Keywords Aonla, endoglucanase, submerged fermentation, Trichoderma harzianum. Top |